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Перевод: electrophoresis

электрофорез

Тезаурус:

1. Polypeptides in affinity-purified DRTF1/E2F (about 5g from about 5x10 10 F9 EC cells) were separated by SDS-polyacrylamide gel electrophoresis and stained with Coomassie blue (track 2); track 1 shows M r standards.
2. SDS gel electrophoresis and western blotting were done as described.
3. In electrophoresis used on a laboratory scale, the separation takes place slowly in a gel that prevents convection currents mixing up the products.
4. The crystallization of enzymes, the invention of the electrical method called electrophoresis which separates molecules according to their size and their electric charge, the development of very high speed centrifuges in which the larger proteins are separated from smaller ones as they spin, and later the application of X-ray crystallography to large molecules have all contributed to progress.
5. A GROUP of biochemists at the Atomic Energy Research Establishment in Harwell has adapted the technique of electrophoresis so it can be used in industry.
6. Proteins were digested directly, or after reduction, alkylation and desalting (), or after reduction and purification by gel electrophoresis ().
7. Here, we report on the combined use of preparative pulsed-field gel electrophoresis (PFGE) and interspersed repetitive sequence (IRS) polymerase chain reaction (PCR) amplification to isolate two DNA markers from a 660 kb Sfi I fragment that contains locus DXS14 and maps to a region between disease-specific incontinentia pigmenti type 1 (IP1) chromosome translocation breakpoints (1).
8. Reaction mixtures were subjected to electrophoresis through polyacrylamide gel under non-denaturing conditions.
9. The new separator relies on electrophoresis: different molecules are pulled off in different directions toward positive or negative electrodes.
10. Cells transformed with plasmids directing the expression of GST-protein A fusion proteins containing one (a), two (b, pAGEX2T) or three (c) IgG binding domains were grown in the presence of 0.1 mM isopropyl--SD-thiogalactopyranoside (IPTG), and proteins analysed by electrophoresis through 10% SDS-polyacrylamide gels followed by transfer to nitrocellulose at 4C in 25 mM Tris-HCl (pH 8.3), 192 mM glycine and 20% v/v methanol.
11. Purified Dcm or Dcm/Cys177Ser protein was incubated with non-radioactive DNA duplexes and analysed by electrophoresis through a 10% polyacrylamide gel containing 2% SDS.
12. This is often achieved by creating heteroduplexes between the two alleles and looking for modified properties in: ( a ) chemical cleavage reactions; ( b ) ribonuclease digestion (one fragment is RNA); ( c ) denaturing gradient gel electrophoresis; ( d ) single strand conformational polymorphism; ( e ) reaction with carbodiimide; or ( f ) automated total sequencing of both fragments.
13. New products for molecular biology research included a pipette for DNA amplification procedures and the Bioprobe Systems' products for hybridisation, electrophoresis and DNA sequencing, chromogenic reagents and specialist membranes.