p
pa
pb
pc
pd
pe
pf
pg
ph
pi
pk
pl
pm
pn
po
pp
pr
ps
pt
pu
pv
pw
px
py
Перевод: preparative
[прилагательное] приготовительный; подготавливающий; [существительное] приготовление [существительное]
Тезаурус:
- Southern blot analysis of the flanking lanes of a preparative PFGE gel containing Sfi I-digested cell line GM06318 (lane 1) and RH 128 (lane 2) DNA; a single filter was sequentially hybridized with probes p58-1, 30C E 2.8, and total human and total hamster DNA.
- These results suggested that most of the IRS-PCR products recovered from preparative slice F DNA were derived from partially digested restriction fragments of the expected size range.
- Probe p58.1 detected a similar 660 kb fragment in both lanes within a region corresponding to preparative gel slice F. Probe 30C E 2.8 recognized a similar 400 kb fragment in both lanes.
- Typically, each chapter gives a brief introduction followed by preparative methods, arranged according to reagent used or building block employed, and concludes with a short section on biological activity.
- To obtain DNA markers from the 660 kb Sfi I fragment, DNA was electroeluted from preparative slice F, extracted, precipitated, and subjected to IRS-PCR (3).
- The preparative portion of the gel was sliced into 14 serial 5-mm sections (A through N; Figure 1); preparative slice F contained DNA fragments ranging from 600 kb to 670 kb in size and included the Sfi I restriction fragment containing DXS14.
- Here, we report on the combined use of preparative pulsed-field gel electrophoresis (PFGE) and interspersed repetitive sequence (IRS) polymerase chain reaction (PCR) amplification to isolate two DNA markers from a 660 kb Sfi I fragment that contains locus DXS14 and maps to a region between disease-specific incontinentia pigmenti type 1 (IP1) chromosome translocation breakpoints (1).
- The preparative portion of the gel containing Sfi I-digested RH 128 DNA was cut into 5 mm gel slices (A-N).
- PCRs were 100 l in volume and contained 5 ng of preparative slice F human DNA, 50 mM KCl, 10 mM Tris-HCl, 1.6 mM MgCl 2 , 0.01% gelatin, 250 M each dNTP, 1 g RNase A, and 0.5 M primer A-TC65N (3, 4).
- The range of sizes include analytical, semi-preparative and preparative dimensions.
- Extensive experience in HPLC solvents and Chromatography Silica enables us to bring together this range which includes both Polymeric and Silica based columns and cartridges, high purity solvents for HPLC Sorbsil silicas for preparative chromatography and Flash chromatography columns.
- LC were purified to 98% I-A + cells by preparative flow cytometry (a, continuous line).
|