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Перевод: sulfate
[существительное] сульфат
Тезаурус:
- Site effects are well-defined in crystals, but are by no means confined to regular solids, and may be observed even in melts, as in molten mixtures of alkali sulfates, where the random distribution of M + ions around the sulfate anions may constitute a low-symmetry environment 8.
- Methylation protection by dimethyl sulfate (DMS) is a powerful and widely used chemical method to probe DNA-protein contacts.
- Mitoplasts were incubated 30 min at 37C. with the lysis buffer (NaCl 0.1M, Tris 10mM pH 7.4, EDTA 1 mM, Proteinase K 100 g/ml, Sodium dodecyl sulfate 1%, RNAsine (Boehringer) 100 u/ml).
- NC, nucleocapsid; HIV-1, human immunodeficiency virus type 1; PCR, polymerase chain reaction; LTR, long terminal repeat; RT, reverse transcriptase; SDS, sodium dodecyl sulfate; PAGE, poly acrylamide gel electrophoresis; EDTA, ethylene diamine tetraacetic acid; IN, integrase; PBS, tRNA primer binding site; MoMuLV, Moloney murine leukemia virus; AMV, avian myeloblastosis virus; nt, nucleotide.
- Lanes 1-;4: samples after precipitation with ammonium sulfate.
- For dimethyl sulfate (DMS) interference, the corresponding oligonucleotides were radioactively labelled, treated for 1 minute with 1% DMS, the DNA was precipitated and complexed with F9 nuclear extract.
- After removal of Alu probes, the filters were also hybridised to nick translated 3 2 P-labelled HPV DNA (210 8 cpm/p) under stringent conditions (50% formamide, 10% dextran sulfate, 5Denhardt's solution, 1% SDS-1 M NaCl, 50 mM TRIS-HCl, pH 7.4, 500 g/ml depurinated salmon sperm DNA) at 42C for 24 hours.
- For the sulfate ion, the triply-degenerate modes can show splitting into two or three components.
- For example, the totally-symmetric stretching mode of the sulfate ion is IR inactive in an environment of high symmetry, as in solution; it becomes active in crystalline phases in sites of lower symmetry.
- Residual proteins are often removed by enzymatic digestion using sodium dodecyl sulfate (SDS) /proteinase K, but the physical separation of the proteins from the nucleic acids is accomplished by centrifugation of a mixture of the nucleic acid solution and phenol and chloroform.
- Briefly, DNA was extracted and incubated with sodium dodecyl sulfate (SDS) and RNase at 37C for three hours.
- We can distinguish monodentate from bidentate sulfate, or terminal from bridging CO ligands, and we can use variations in the CO stretching frequencies in metal carbonyls to make deductions about the electronic natures of the other ligands.
- Protein dissociation buffer (10% -mercaptoethanol, 10% sodium dodecyl sulfate SDS, 25% glycerol, 10 mM Tris hydrochloride pH 6.8, 0.02% bromophenol blue) was added to each sample, and the mixture was incubated at 100C for 5 min.
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