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Перевод: absorbance

[существительное]
спектральная поглощательная способность

Тезаурус:

1. A wide choice of spectrophotometric methods (absorbance, reflectance, fluorescence, luminescence) and geometry (single optical fibre, bundle, bifurcated fibre) is feasible; all that is needed is that the biolayer modulates the light path.
2. To the left are shown examples of individual absorbance spectra for the three types of cones: the max values lie close to 565, 535 and 430nm, as is typical for Old World primates.
3. Stock chymotrypsin solution (Sigma; product code C4129) was made up in HCl (25mmoldm -3 ) and the concentration adjusted with TES (100mM, pH7.8) to produce a rate of change of absorbance of 0.025 units min -1 at 405nm and 20C in a control assay consisting of chymotrypsin (7-;8g in 0.6ml); 0.3ml TES (100mM, pH7.8); and 0.3ml of a solution of substrate (1.2mM) in 50% dimethylsulphoxide/TES.
4. For each individual record, the max was estimated by fitting a template to the long-wave limb of the absorbance spectrum.
5. The Series 3000 incorporates automatic calibration of wavelength, transmission and absorbance zeros together with automatic programmes for absorbance ratioing and peak seeking.
6. The absorbance spectra of individual receptors were measured with a modified dual-beam Liebman microspectrophotometer under computer control; the numerical apertures of the condenser and objective were 0.4 and 0.9, respectively.
7. The reaction was stopped by addition of 800l of 0.25M NaOH and the absorbance at 410nm ( A 410 ) was measured.
8. - Galactosidase (- gal) co-transfections were analysed by a colorimetric assay (Promega), in which 1 unit - galactosidase activity is represented by one absorbance unit at 410nm.
9. Note that we have included in this sample some records that would not pass our standard criteria if our purpose were to establish the exact form of the mean absorbance spectrum.
10. To minimize the effects of bleaching, only one absorption spectrum was usually obtained from a given cell, but two independent estimates of the baseline absorbance spectrum were always obtained by arranging both beams to pass outside the cell.
11. In the case of cells that absorbed at short wavelengths, we checked that the pigment was photolabile by repeating the absorbance measurements after bleaching the cell with white light for 5min.
12. Relative -galactosidase units (U) in cultures induced for 12h with galactose were measured as described and normalized to cell numbers as U=1,000A 420 (CVt) -1 , where A 420 is the absorbance at 420nm, C is the density of the cell suspension (in A 600 ml -1 ), V is the volume of cell suspension (ml), and t is the total incubation time (min).