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Перевод: assay
[существительное] испытание; проверка ; проба ; проба металлов; опробование; анализ ; количественный анализ; образец для анализа; [глагол] испытывать; опробовать; проводить количественный анализ; пробовать
Тезаурус:
- In the in vivo assay both mutations behaved as the wild type, but they had slightly lower affinities than wild type in vitro as judged by gel retardation assays.
- The HA-2-specific CTL clone, designated HA-2 (ref. 25), and the HLA-A2.1 alloreactive CTL clone were mixed with chromium-labelled targets at effector-to-target ratios of 10:1 and 1:1, and specific lysis was measured in a 4-h chromium release assay; values represent per cent specific lysis.
- Cell extracts (0.3g protein per assay) were incubated for 30min with phospholipid vesicles containing PtdIns(4,5)P 2 at the concentrations of free Ca 2+ indicated at the abscissa.
- FIG. 1 HLA assembly assay.
- The assay was modified from ref. 13.
- Cells from donor Z 22 were initially tested (assay 1) on five different sets of (1-;4) peptides as shown, using as targets a BCL HLA-matched to the donor only for HLA-B35.
- (L) and c.p.m. (0) represent the amount of bound 125 I-labelled substance P in the presence or in the absence of unlabelled ligand, respectively; L represents the concentration of unlabelled ligand, and IC 50 represents the concentration of unlabelled ligand that causes 50% inhibition of specifically bound 125 I-labelled substance P. Either Bolton-Hunter 125 I-labelled substance P or 125 I Tyr8-substance P were used in the assay; both radioligands gave identical IC 50 values.
- As Cocking (1989) points out, many of these traits would ultimately be revealed in conventional plant breeding programmes but tissue culture provides a much more rapid means of assay.
- The assay was done for 30 min in the presence of either 0.1M free Ca 2+ and 0.9mM sodium deoxycholate (+), or 10M free Ca 2+ and 3.4mM sodium deoxycholate (++).
- In assay 2, these peptides and cp29, which are four allelic variants (Table 2), were tested individually, again at an effector to target cell ratio of 50:1.
- Transfection and RNAse protection assay
- Lysis was determined in a 5-h chromium-release assay at effector-to-target ratios ranging from 10:1 to 0.3:1.
- The Necromundan neophytes became very familiar with the operating altars and biomonitoring and chemical assay machines of the Apothacarion - with the mantis-like laser scalpels, the stasis tureens cradling the precious new organs, the examinator device towering like a brass-banded armadillo, its tapering snout scanning the innards of the body, and with the soporificator instrument resembling some giant spider that stung metacurare into the nerves; to which the drone of surgical incantations from the adepts, whose robes were embroidered with arabesque prophylactic hexes and purity emblems, was a macabre lullaby.
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