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Перевод: assayed

производил анализ


Тезаурус:

  1. In the experiments shown in Table 1, pin activity was assayed by the inhibitory effect of leaf extracts on chymotrypsin activity (Table 1 legend).
  2. MIP-1 (1:100) or control conditioned medium was added to plastic co-immobilized protein for 30min, free cytokine was then washed out and adhesion was assayed as for Fig. 2.
  3. Supernatants were diluted 200-fold with buffer A containing no EDTA and 18.5mM sodium cholate and assayed for PLC activity as described, except that EGTA (3mM) was used as a chelating agent.
  4. Detergent-released DRTF1/E2F was further assayed for reactivity with anti-peptide A (antiserum 3; tracks 6 and 7) and anti-peptide 18 (antiserum 11; tracks 8 and 9) in the presence of either unrelated peptide 1 (tracks 6 and 8) or the homologous peptides (tracks 7 and 9).
  5. DNA-binding polypeptides in affinity-purified DRTF1/E2F were assayed by crosslinking to the adenovirus E2A promoter distal E2A site (binding site details indicated at bottom right) either in the absence (track 4) or presence of competing wild-type (track 5) or mutant (track 6) E2F binding sites; M r standards shown in track 3.
  6. b and c , Adhesion was assayed as described.
  7. The purified protein was assayed at the indicated concentrations for its ability to hydrolyse p- nitrophenol phosphate (pNPP) either in the absence () or presence () of 200M sodium vanadate.
  8. c , Wild-type Myc and the indicated mutant derivatives were assayed for transactivation of SRE-CYC1-LacZ through dimerization with SRF-Max72 and of CACGTG-CYC1-LacZ through dimerization and DNA binding with max.
  9. Free cytokine was then removed by washing the plates 5 times in PBS; 51 Cr-labelled CD8 + T cells were added to the plates and the number of cells binding was assayed as described in Fig. 1 legend.
  10. Proliferating Rat-1/c-Myc-ER/Bcl-2 fibroblasts and control cells were then assayed for c-Myc-induced apoptosis by microscopic inspection 48h after serum depletion.
  11. The cytoplasmic inner leaflets of the plasma membranes of erythrocytes and quiescent platelets have high procoagulant activities when they are assayed in vitro .
  12. Innervation was assayed with anti-horseradish immunocytochemistry (Fig. 1 legend) following recording.
  13. The amount of prostacyclin generated (assayed by inhibition of ADP-induced aggregation) by vascular tissue was significantly reduced in these mildly diabetic animals (Silberbauer et al, 1980).

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